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KMID : 1100720180380060569
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Annals of Laboratory Medicine
2018 Volume.38 No. 6 p.569 ~ p.577
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Detection of Rifampicin- and Isoniazid-Resistant Mycobacterium tuberculosis Using the Quantamatrix Multiplexed Assay Platform System
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Wang Hye-Young
Uh Young
Kim Seo-Yong
Cho Eun-Jin
Lee Jong-Seok
Lee Hye-Young
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Abstract
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Background: The increasing prevalence of drug-resistant tuberculosis (TB) infection represents a global public health emergency. We evaluated the usefulness of a newly developed multiplexed, bead-based bioassay (Quantamatrix Multiplexed Assay Platform [QMAP], QuantaMatrix, Seoul, Korea) to rapidly identify the Mycobacterium tuberculosis complex (MTBC) and detect rifampicin (RIF) and isoniazid (INH) resistance-associated mutations.
Methods: A total of 200 clinical isolates from respiratory samples were used. Phenotypic anti-TB drug susceptibility testing (DST) results were compared with those of the QMAP system, reverse blot hybridization (REBA) MTB-MDR assay, and gene sequencing analysis.
Results: Compared with the phenotypic DST results, the sensitivity and specificity of the QMAP system were 96.4% (106/110; 95% confidence interval [CI] 0.9072?0.9888) and 80.0% (72/90; 95% CI 0.7052?0.8705), respectively, for RIF resistance and 75.0% (108/144; 95% CI 0.6731?0.8139) and 96.4% (54/56; 95% CI 0.8718?0.9972), respectively, for INH resistance. The agreement rates between the QMAP system and REBA MTB-MDR assay for RIF and INH resistance detection were 97.6% (121/124; 95% CI 0.9282?0.9949) and 99.1% (109/110; 95% CI 0.9453?1.0000), respectively. Comparison between the QMAP system and gene sequencing analysis showed an overall agreement of 100% for RIF resistance (110/110; 95% CI 0.9711?1.0000) and INH resistance (124/124; 95% CI 0.9743?1.0000).
Conclusions: The QMAP system may serve as a useful screening method for identifying and accurately discriminating MTBC from non-tuberculous mycobacteria, as well as determining RIF- and INH-resistant MTB strains.
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KEYWORD
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Mycobacterium tuberculosis complex, Rifampicin, Isoniazid, Drug susceptibility testing, Quantamatrix Multiplexed Assay Platform
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